Fungal infections are treated by administering a combination of (a) amphotericin B, and (b) a glycerol ether selected from the group consisting of (i) HOCH.sub.2 CHOHCH.sub.2 OR, (ii) HOCH.sub.2 CH(OR.sub.1)CH.sub.2 OH, and (iii) combinations thereof, wherein R and R.sub.1 are independently selected from the group consisting of C.sub.8 -C.sub.18 and C.sub.8 -C.sub.18 alkenyl. The glycerol ether acts synergistically to reduce the minimum inhibitory concentration of amphotericin B. The combination is particularly effective against Cryptococcus and Candida species.