|United States Patent||3,964,865|
|Das||June 22, 1976|
A preweighed vial of lyophilized methemoglobin (ferrihemoglobin) provides a simple, stable, reproducible standard for colorimetric determination of total hemoglobin concentration in whole blood by the cyanmethemoglobin method. Conversion of the standard and of hemoglobin in blood samples to cyanmethemoglobin is accomplished with Drabkin's solution reconstituted from a dry powder and having an added non-ionic surface active agent (Brij-35). Color intensity is measured at 540 m.mu..
|Inventors:||Das; Manik L. (Crestwood, MO)|
Sigma Chemical Company
|Filed:||January 30, 1975|
|Current U.S. Class:||436/66 ; 356/39; 356/415; 356/42; 436/15; 436/18|
|Current International Class:||G01N 33/96 (20060101); G01N 021/20 (); G01N 033/16 ()|
|Field of Search:||23/23B 426/384,385 252/408 424/7 356/39,42|
harvey A. Itano et al., Medicine, 35, 131 (Feb. 1956). .
Todd-Sanford Clinical Diagnosis, I. Davidson et al., eds., 14th Edition, 139, W. B. Saunders, Phila., 1969. .
Gradwohl's Clinical Laboratory Methods, Sam Frankel et al., eds., vol. I, 396-406, C. V. Mosby Co., Saint Louis, 1970..