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United States Patent | 5,001,050 |
Blanco , et al. | March 19, 1991 |
An improved method for determining the nucleotide base sequence of a DNA molecule. The method includes annealing the DNA molecule with a primer molecule able to hybridize to the DNA molecule; incubating the annealed mixture in a vessel containing four different deoxynucleoside triphosphates, a DNA polymerase, and one or more DNA synthesis terminating agents which terminate DNA synthesis at a specific nucleotide base, wherein each the agent terminates DNA synthesis at a different nucleotide base; and separating the DNA products of the incubating reaction according to size, whereby at least a part of the nucleotide base sequence of the DNA can be determined. The improvement is provision of a DNA-polymerase which is a .phi.29-type DNA polymerase.
Inventors: | Blanco; Luis (Madrid, ES), Bernad; Antonio (Madrid, ES), Salas; Margarita (Madrid, ES) |
Assignee: |
Consejo Superior Investigaciones Cientificas
(Madrid,
ES)
|
Appl. No.: | 07/328,462 |
Filed: | March 24, 1989 |
Current U.S. Class: | 435/5 ; 435/183; 435/194; 435/6; 435/91.2; 435/91.5; 436/501; 436/93 |
Current International Class: | C12Q 1/68 (20060101); C12N 9/12 (20060101); C12Q 001/70 (); C12N 009/12 (); G01N 033/566 (); C12P 019/34 () |
Field of Search: | 435/6,91,194,810,5 436/501 935/77,78 |
4795699 | January 1989 | Tabor et al. |
4921794 | May 1990 | Tabor et al. |
4942130 | July 1990 | Tabor et al. |
4946786 | August 1990 | Tabor et al. |
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